Evaluation of Anti Cataractogenic Activity of Cerium Oxide Nanoparticles in Corticosteroid Induced Cataract

Author(s): B. Nagaraju*, Y. Kranti, K. Prasad, K. Chandrasekar4 and SK. Manesh

Abstract: Aim: To investigate the anti cataractogenic activity of Cerium oxide nanoparticles on Prednisone induced cataract by using isolated goat lens. Methods: Anti cataract activity is done by using isolated goat lens. Goat lens were divided into four groups. Group I lens were incubated in artificial aqueous humor (normal control). Group II lens were incubated with Prednisone 100mg (toxic control). Group III and IV lens were incubated with Prednisone and Cerium oxide nanoparticles (10mg and 20mg) and subjected to photographic evaluation for opacity, lens was homogenized by using tris phosphate buffer and sodium, potassium, total protein and catalase concentrations were determined Results: The grades of opacity was 0,3,1 in group I, II and III respectively. The present study showed higher total proteins (P < 0.05 at all concentration) and K+ ions (P<0.05 at all concentration) whereas lower concentrations of Na+ ions (P<0.05 at all concentration) with Cerium oxide nanoparticles treated groups. The level of Catalase was found to be less in experimentally induced cataract lenses as compared to normal control group. The lenses treated with Cerium oxide nanoparticles showed significant rise in enzyme level suggesting maintenance of antioxidant enzyme integrity. Conclusion: The Present investigation suggests that Cerium oxide nanoparticles effectively prevent the cataractogenic condition. Thus, the goat lens model and prednisone induced cataract model could be used for testing of various anti cataract agents.

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