Study on Anti-inflammatory, Anticoagulant, Antiplatelet Activity of Grewia serrulata DC. and Grewia heterotricha Mast

Author(s): Usha B, Sharath Kumar M Nandish, Shashidhar Bharadwaj. S, Thejaswi N, Devaraja Sannaningaiah*, Pushpalatha K. C

Abstract: Aim: To evaluate anti-inflammatory, anticoagulant, antiplatelet activity of aqueous leaf extract of Grewia heterotricha Mast.and Grewia serrulata DC. Methods: The aqueous leaf extract of these plants were assessed for their in-vitro anti-inflammatory activity by inhibition of albumin denaturation, Protein inhibitory action, heat- induced hemolytic activity methods and in-vivo anti-inflammatory activity by Carrageenan Induced Rat Paw Edema method. Primary phytoconstituents were screened by RP-HPLC, FTIR and GC-MS. Results: Aqueous extracts of both the plants showed promising anti-inflammatory activity by inhibiting protein denaturation with IC50 values 491.4μg/ml (AEGH) and 742.6μg/ml (AEGS). HRBC membrane stabilizing assay of AEGH and AEGS showed significant inhibition values of 56.26±0.37% and 55.03±0.37% respectively. Proteinase inhibitory activity of AEGH and AEGS was found to be 66.01±0.12% and 54.83±0.02%respectively. In addition, presence of different functional groups was adjudged by FTIR and primary phytoconstituents were screened by RP-HPLC and GC-MS. Furthermore, AEGH and AEGS prolonged the citrated human plasma in both PRP (Platelet Rich Plasma) and PPP (Platelet Poor Plasma) from control 190s to 290s, 270s to 420s, 150s to 250s and 260s to 410s respectively. This confirms the anticoagulant nature of AEGH and AEGS. Moreover, both AEGH and AEGS did not alter ADP induced platelet aggregation suggested that they have no role on platelets. Conclusion: The aqueous leaf extract of Grewia serrulata DC. (AEGS) and Grewia heterotricha Mast.(AEGH) have promising anti-inflammatory and anti-coagulant activity. The active components in the extracts may be responsible for these properties. Isolation of the active components might help in the field of anti-inflammatory drug research.

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