Morphological and Molecular Diversity Analysis in Cluster Bean [Cyamopsis tetragonoloba(L.) Taub].

Author(s): Rama Dadheech*, R. Sharma, H. R. Mahla And R. K. Bhatt

Abstract: A study was conducted on thirty-four genotypes of cluster bean to find out the morphological and molecular diversity. Fifteen morphological characters revealed 0.19 average diversity (Manhattan’s) with the range from 0.06 to 0.41. RAPD generated a total of 77 amplicons of which only 25 were polymorphic whereas, ISSRs generated 52 amplicons detecting no polymorphisms. RAPD based (Jaccard’s) dissimilarity coefficient ranged from 0.01 to 0.24 with the mean genetic diversity of 0.10. Polymorphism information content (PIC) for RAPD, with an average of 0.05, ranged from 0.0 to 0.26. The dissimilarity matrix generated for morphological traits was positively correlated with RAPD dissimilarity matrix (r = 0.29). Vegetable types (M-83 and HVG2-30) were more diverse with each other and from other genotypes Varieties developed at Jhansi and Punjab were closer to Gujrat varieties, indicating their origin from Gujrat material. AMOVA showed higher diversity within (83%) than among (17%) populations.

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