Isolation and Identification of Endophytic Fungi from Tree Bark Showing Potent Antioxidant Activity

Author(s): Khandelwal Sharad R*, Kedar Jyoti A and Bholay Avinash D

Abstract: Endophytes are mutualistic harbors inside the living tissues of healthy plants without causing any symptoms of infection. These are important source of bioactive molecules, which have broad range of biological activities. An endophytic fungus isolated from bark of Tamarindus indica, Ficus benghlensis and Mangifera indica. The fungus was identified as Aspergillus spp.1, Cladosporium spp. and Aspergillus spp.2 based on morphological characterization. The ethyl acetate cell free extract was qualitatively studied by using TLC, which showed Rf values for phenol standard 0.45, and 0.45,0.47,0.42, for samples A1, C and A2 respectively, and for flavonoids standard was 0.82,for one sample i.e. Aspergillus spp.1 showed Rf value 0.85.Total phenol content by Folin-catetu method, among three extract highest concentration ,98.58 μg/mg,other two showed 83.90 and 70.70 μg/mg.Total flavonoids by AlCl3 method, the total flavonoids content in Aspergillus spp.1 was found to be 90.10 μg/mg. protein. All three extracts showed DPPH radical scavenging and H2O2 Scavenging property. Aspergillus spp. 2 (Mangifera indica) showed maximum % inhibition as compared to Ascorbic acid, Aspergillus spp. 1from (Tamarindus indica) and Cladosporium spp. from (Ficus benghalensis). The present study suggests that endophytic fungi associated with tree bark are potential agent as antioxidant activity.

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