In Vitro Evaluation of Anti-inflammatory Activity in Seed and Callus Extract of Trigonella foenum-graecum

Author(s): Babita Rana*

Abstract: Trigonella foenum gracecum is well known medicinal spice worldwide because of its alluring therapeutic properties. Investigations were executed to study in vitro anti-inflammatory activity in seed and callus extract of Trigonella foenum-graecum. Callus was induced from seed explants in MS medium supplemented with 2, 4- D. (2mg/l). Enough callus was obtained after 4 weeks of inoculation of explants. Crude extracts of seed and callus prepared in chloroform were evaluated for their anti-inflammatory activity by inhibition of Albumin denaturation and HRBC membrane stabilization method. Callus extract showed more inhibition of Albumin denaturation and heat induced hemolysis of HRBC membrane as compared to seed extract. The anti-inflammatory activity can be attributed to various phytochemicals such as flavonoids, polyphenols, triterpenoids, steroids and alkaloids. The finding suggests the potential therapeutic use of callus of Trigonella foenum-graecum for its anti-inflammatory activity.

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