Multiplex PCR: Improving Microbiological Diagnosis, A Review

Author(s): Garba, I. and Udefi, A.C.,Dept. of Medical Microbiology, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.

Abstract: nucleic acid segments present in small or complex samples to sufficiently high levels to allow their detection by optical, physical and other methods. It is a rapid and simple way of copying specific DNA fragments from minute quantities of source DNA material using a heat-stable polymerase and suitable primers to direct the amplification of the desired region of DNA. Objective: The objective is to review multiplex PCR and its application in diagnosis of disease. Method: The method adopted in this review is broad search of both electronic data bases, hard copies by studying and comparing the information obtained from available current published scientific researchers in peer reviewed journals, books, and conferences etc. Results: it was found that Diagnostic microbiology is in the midst of a new era. Rapid nucleic acid amplification and detection technologies are quickly displacing the traditional assays based on pathogen phenotype rather than genotype. Conclusion: The review concludes that PCR technology is gradually changing the world of rapid laboratory diagnosis.

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