In Vitro Propagation of Yellow Color Capsicum annuum

Author(s): Aishwarya Sangle, Neha Pawar, Komal Shejwal and Manisha Chalse*

Abstract: A simple and efficient protocol was developed for in vitro propagation of yellow color capsicum annuum. Seeds of capsicum annuum were decontaminated and placed in MS medium and kept it for germination. Leaf explants excised from one-month old aseptic seedlings and were cultured on MS medium containing different hormone with different concentrations and combinations of auxins and cytokines. The highest callus was obtained from leaf explants with NAA(2.0mg/L) and with kinetin(5.0mg/L). Calli derived from leaf explants were transferred to the shooting media. The media containing(2.5.mg/L) had the best shoot regeneration. Regenerated plants with six to eight leaves were potted protected by gaze and kept about one week in the culture room. Then transferred to the green house and grown to maturation.

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