Study on Phytochemical Characterization and Antibacterial Activity of Fruit Trees of Chamoli District, Uttarakhand, India

Author(s): Maneesha Singh*and Rakhi Purohit

Abstract: Some of important local fruit trees of Uttarakhand, India such as Rubus ellipticus, Berberis aristata, Zizipus mauirtiana showed astringent and febrifuge properties etc. Ethanobotanically, fruit juices of these plants are sore and used to treat skin diseases, hypertension, cancer, malaria, tranquilizer etc. In continuation of these efforts in search of various biological activities, present study deals with estimation of phytochemical constituents of Rubus ellipticus, Berberis aristata, Zizipus maurntina and screened their leaves for their antimicrobial activity. Phytochemical screening of the ethanol extract showed the presence of carbohydrates, tannins and phenolic in all the studied plant samples whereas alkaloid is present in Berberis aristata and Zizipus maurintina, and cardiac glycosides was present only in Rubus ellipticus. The extract showed potent results against six bacterial strains, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Bacillus cereus. Out of these studied plants, Rubus ellipticus has highest zone of inhibition and maximum activity against E. faecalis and lowest against E. coli. MIC analysis was performed by dilution of the concentrated ethanol extract in pure DMSO to achieve a decreasing concentration of 1000μg/ml to 62.5μg/ml. On performing MIC analysis for the ethanol extract of Rubus ellipticus, the results showed highest activity against E. facecalis (16mg/ml) at 1000μg/ml concentration and minimum of Salmonella typhi (10mg/ml). So, it can be concluded that ethanol extract of fruit plants possesses good antibacterial activity that can be enhanced if the phytoactive components are purified and adequate dosage is determined for proper administration.

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