A Simple and Improved HPLC Method Development and Validation and Stability Studies for Estimation of Ropinerole in Tablet Dosage Forms

Author(s): A. Manasa*, P. Rani, B. Sharanya and T. Surakshita

Abstract: A simple and accurate, precise method was developed for the estimation of the Ropinerole in bulk and pharmaceutical dosage form. The chromatography was performed by running through X-Bridge C18 Column which is having 250X4.6 mm I.D with 5 μm particle size. Column temperature was maintained at ambient, with a mobile phase phosphate buffer: Acetonitrile at the ratio of 60:40 v/v. The flow rate was 1 ml/min and the UV detections was carried out at 210-300 nm. Retention time of Ropinerole were found to be 2.29 mins. The percentage purity of the Ropinerole were found to be 99.76% respectively. The average percentage recovery of Ropinerole was found to be 98.94%. The LOD values of Ropinerole was 0.03 μg/ml and LOQ values were 0.09 μg/ml. The intraday and inter day precision (%RSD) was found to be 0.86 & 0.87 and repeatability was found to be 0.440 less than, the method was validated as per ICH guidelines. The percentage recovery was in good agreement and the method is simple, specific, precise, and accurate for the determination of Ropinerole which can be applied for the routine quality control analysis. The statistical parameters and recovery studies were carried out and reported.

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