RP-HPLC Method for The Simultaneous Estimation of Lumefantrine and Artemether in Pure Form and Tablet Dosage Form

Author(s): Swapna Mamindla and Srinivas Ampati*

Abstract: A new, simple, accurate, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the separation and quantification of Lumefantrine and Artemether in pure form and tablet dosage form. The determination was carried out using Symmetry C18 ODS (4.6mm×250mm, 5μm) particle size as a stationary phase and mobile phase comprised of Methanol: TEA Buffer (36:64v/v) and the pH of tri ethyl amine buffer adjusted to pH-4.2 using orthophosphoric acid. The flow rate was maintained at 1.0 ml/min and the eluent was monitored at 296nm.The retention time of Lumefantrine and Artemether were 2.249 min and 5.430min respectively. The method was validated in terms of linearity, precision, accuracy, specificity and robustness. The method was linear and for precision studies; RSD for RIS AND HPD were 0.02 and 0.04 respectively. The percentage recoveries for both drugs from their tablets were 100.2203 and 100.60% respectively. The method precision for the determination of assay was below 2.0 %RSD. The method is useful in the quality control of pharmaceutical formulations.

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