Simultaneous Quantitative Estimation for the Method Development, Validation and Stability Studies of Lesinurad and Allopurinol in Bulk and Pharmaceutical Dosage Form by RP-HPLC

Author(s): B. Preethi*, M. Anuradha and R. Shyamsunder

Abstract: Objective: The objective of the present research work was to develop an innovative, simple, and economic method for estimation of Lesinurad and Allopurinol in bulk and dosage form by RP-HPLC. Methods: The chromatographic conditions were performed on Phenomenex Luna C18, 100A, 5μm, 250mmx4.6mm i.d.as stationary phase and mobile phase was prepared with a mixture of Phosphate buffer (pH – 3.9): Acetonitrile flow 1.0 ml/min, with Injection Volume 20μl, at detection wavelength 252 nm and run time at 6.0 min. Results: The analytical method is valid for estimation of Lesinurad and Allopurinol over a range of 0-150 μg/ml, 0-15 μg/ml. The results of system suitability test, linearity, precision and accuracy, robustness, specificity, LOD and LOQ and stabilities presented in this report are within the acceptance range, Retention time of Lesinurad and Allopurinol was found to be2.246 and 3.132. Conclusion: A specific, sensitive, economic method estimation of Lesinurad and Allopurinol has been developed based on ICH Guidelines with bulk and dosage forms.

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