In vitro and in vivo Evaluation of Ivabradine Loaded Microsphres Prepared by Multiple Emulsion Solvent Evaporation Method

Author(s): Firke Sagar N * and Ghiware Nitin B

Abstract: The Present study is an attempt to avoid the repetitive administration and release of drug in a controlled manner, thereby improve the bioavailability. Ivabradine HCl Floating microspheres were prepared by multiple emulsion solvent evaporation technique using Ethyl Cellulose as polymer and Tween 80 as emulsifying agent. During process optimization various parameters were studied such as: drug: polymer ratio and stirring speed. Selected optimized formulations were studied for SEM, entrapment, floating behavior, drug release and kinetics. Microspheres prepared were spherical shaped with smooth surface. Size of microspheres was in the range of 137.40 μm to 183.23μm. Formulation prepared using 400 mg of Ethyl cellulose gives the highest yield of 89.10±10 %, 91.5±0.10 % of drug loading, and 78.20±0.27 percent of drug release and 92.10±0.26 of Buoyancy. In vivo floating ability (X-ray) study, in-vivo Cardioprotective activity was performed on rats. Formulation remained buoyant for more than 8 h. Optimized formulation treated group shows significant increase in Bioavailability. Ivabradine loaded floating microspheres expected to give new choice for safe, economical and increased bioavailable formulation for effective management of Angina Pectoris.

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