Formulation Development and Evaluation of Ciprofloxacin HCl Transfersomes

Author(s): Abdul Mannan and Wajeeda Begum

Abstract: Ciprofloxacin hydrochloride (CIP HCL) is broad spectrum antibiotic belongs to fluoroquinolones which is active against both gram-positive and gram-negative bacteria. It has low permeability. The aim of the present study is to formulate CIP loaded Transferosomes to overcome the barrier function of the skin and enhance the permeability. CIP loaded Transferosomes was optimized using 23 full factorial design using sigma tech software, were independent variables are Span80 (X1), Tween80 (X2) and Phospholipid as (X3) and the responses are entrapment efficiency (Y1), particles size determination (Y2) and in vitro drug release (Y3). In the Preformulation studies it was proven that the drug solubility in 7.4 pH was 1820.22±0.151 mcg/ml, and FTIR shows no interaction between drug and excipients used. Thin film hydration technique is use for preparation of Transfersomes. It was concluded that the factorial design (23) by composite method total of 18 formulations was carried out which had the ability to obtain an optimized CIP HCL Transfersomes with maximize EE% (88.97±0.31), minimize particle size (90.1±0.21), and maximize transdermal flux (5.341±4.21). SEM of optimized CIP Transfersomes appeared as spherical, well identified, unilamellar nanovesicles. After optimization of formulation variables, it was found that the optimized formulation was suggested to contain 525, 525 and 315 mg of X1, X2, and X3, respectively. Therefore, Ciprofloxacin Hcl loaded Transfersomes are successfully optimize for transdermal drug delivery.

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