Formulation, Development and Evaluation of Phytosomal Gel of Thymoquinone

Author(s): Abdul Mannan, Safiya Begum* and Asif Rasheed

Abstract: Phytosomes is vesicular drug delivery system in which phytoconstituents of herb extract surround and bound by lipid (one phyto-constituent molecule linked with at least one phospholipid molecule) Thymoquinone is the main active constituent of nigella sativa oil. Using Design of experiment (sigmatech software) 12 different formulations were prepared. Two different independent variables were used which include: Amount of lipids (X1), Amount of cholesterol (X2). The independent variables were screened using a multilevel factorial design (22) and four different formulations, four mid points and four replicates of Thymoquinone. All the formulations was prepared using thin film hydration method. The Thymoquinone was more soluble in Methanol than in Ethanol, phosphate buffer pH 7.4 and water. The prepared thymoquinone was subject to the %EE, In vitro drug release and particle size. The highest entrapment efficiency was found to be 90.8 ± 0.6 % and In vitro drug release was found to be 94.28 ± 0.39 % which is selected as optimized formulation and SEM was evaluated. 0.2% and 0.4% % gel was prepared taking optimized formulation by using 1% carbopol 934 and evaluated for Homogenicity, spreadability, pH, Viscosity, Drug content and In vitro drug release.

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