Evaluation of Cardioprotective Activity of flowers of Butea monosperma against Isoproterenol induced Cardiac Hypertrophy in Wistar Albino Rats

Author(s): Rajashekar Perusomula* and Ramesh Alluri

Abstract: The objective of the present study was to investigate the effect of pretreatment with Butea monosperma on isoproterenol (ISO)-induced cardiotoxicity and cardiac hypertrophy in rats. Methods: Albino male Wistar rats (250–300 g) were evenly divided into following groups: Control group (2% acacia solution (2ml/kg p.o.) orally for 18 days and water IP from days 9–18). ISO group (olive oil 2 mL/kg orally for 18 days and ISO 1 mg/kg IP from days 9–18); BM (300mg/kg)+ ISO: Animals were administered with BM (300mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). BM (600mg/kg) + ISO: Animals were administered with BM (600mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). Co Q10 (100mg/kg) + ISO: Animals were administered with Co Q10 (100mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). And the twenty-four hours after the last dose of water or ISO, the rats were anesthetized, and an ECG was recorded. Blood was withdrawn by retro-orbital puncture for estimation of serum creatine kinase-MB (CK-MB) isoenzyme levels, lactate dehydrogenase (LDH) levels, and aspartate aminotransferase activities. The animals were euthanized using an overdose of ether. The hearts of 6 animals from each group were used for estimation of superoxide dismutase (SOD) activity, reduced glutathione (GSH) concentration, lipid peroxidation (LPO), malondialdehyde (MDA), and total protein concentration. Results: A total of 30 rats (6 in each group) were used in this study; all rats survived to study end. Compared with the control group, the ISO-treated rats had a significant change in the endogenous antioxidants (ie, significantly higher myocardial MDA concentration [P < 0.001]; significantly lower myocardial GSH concentration [P < 0.001] and SOD activity [P < 0.01]); and significantly higher serum activities of marker enzymes (eg, CK-MB [P < 0.001] and LDH [P < 0.001]). Compared with the ISO group, the BM 300 mg/kg + ISO group had a significant change in the endogenous antioxidants (ie, significantly lower MDA concentration [P < 0.001]; significantly higher myocardial GSH concentration [P < 0.001] and SOD activity [P < 0.001]); and significantly lower serum activities of marker enzymes (eg, CK-MB [P < 0.05] and LDH [P < 0.001]. Conclusion: Pretreatment with BM (1300 mg/kg) for 18 days was associated with moderate protection against ISO-induced cardiotoxicity and cardiac hypertrophy, and with lower myocardial injury by preserving endogenous antioxidants and reducing LPO in rat heart.

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