Phytochemical Characterization of Topoisomerase-II Inhibitor from Freshwater Algae Chlorosarcinopsis eremi (Chantanachat, S. and Bold, H.C)

Author(s): Dhanalakshmi Marimuthu* and Angayarkanni Jayaraman

Abstract: The freshwater algae Chlorosarcinopsis eremi, belong to the family Chlorophyceae. The methanol extract of the algae of the present study were assayed for inhibition of relaxation activity of topoisomerase II. The methanol extract was subjected to thin layer chromatography and column chromatography to separate the phytochemical constituent present in the methanol extract. The obtained fractions were again assayed for inhibition of relaxation activity of topoisomerase II. The inhibition of relaxation activity of topoisomerase II enzyme was found maximum in Fraction II compared to other fractions of methanol extract. From the Gas Chromatography - Mass Spectrometery analyses of methanol fraction II, the present study concluded 2-Allyl-t-Butylhydroquinone may be the active metabolite responsible for Topoisomerase II enzyme inhibition.

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