Analytical Method Development and Validation of Guaifenesin by RP-HPLC Method (Research Article)

Author(s): Gopal Rao. Paisa and Ravali. B

Abstract: A novel very rapid, sensitive, reverse phase High Performance Liquid Chromatography (RP-HPLC) technique was developed for the quantitative estimation of Guaifenesin in bulk and tablet dosage form. It was resolved by using a mobile phase Potassium di-hydrogen phosphate: methanol in the ratio (60:40 v/v) at a flow rate of 0.8mL/min. using UV - Visible detector at the wavelength of 228 nm for quantification. Efficient separation was achieved for Guaifenesin on used Waters Acquity HSS T-3 C18 (100 × 2.1 mm, 5μm). The retention time of Guaifenesin was 2.433min. The calibration graphs were linear and the method showed excellent recovery for tablet dosage form. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness.

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