Isolation and Characterization of Airborne Bacteria from The Hospital Environment

Author(s): Ravikiran Regeti, Raghu Gogada and *Praveen Boddana

Abstract: Microbial flora can be a source of numerous allergic and infective illnesses. Atmosphere is a hostile environment for micro-organisms, this information provides indoor air quality and on associated potential risks in hospitals. Samples were collected from 20 wards by settling plate technique during January-march 2012 in Government Hospital, Pathapatnam, Andhra Pradesh, INDIA. The number of colonies per plate was variable (15-255 colonies). Maximum number of colonies were observed, the organisms isolated during this study were micrococci, aerobic spore bearing bacilli (asb) coagulase negative staphylococci, gram negative bacilli. Micrococci were isolated from 15 wards, ASB from 12 wards, coagulase negative staphylococci from 12 wards, gram Negative bacilli in 2 wards. Antibiotic sensitivity testing was done by Kirby Bauer’s method for coagulase negative staphylococci. They were found sensitive to Gentamicin, Kefbactam, ciprofloxacin, ofloxacin, and cloxacillin in descending order of sensitivity.

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