Alteration of Energy Metabolism in Sodium Selenite Induced Rat Model (Research Article)

Author(s): C. Samson Raju* and G. Swathi

Abstract: Selenium is a metalloid present as selenide minerals, selenite and selenite salts. Selenium has many good benefits as it act as antioxidant, protects DNA damage, supports immune system etc., besides them if the selenium limit exceeds it causes toxic effects. In this contest we investigated the acute toxicity of sodium selenite in rat model by inducing sodium selenite. We had obtained average value of Lethal Dose (LD50) as 7.14mg/kg. Rats were divided into four groups consisted of ten rats in each group. Group (1) as control rats treated with saline, Group (2) as single dose induced Sodium selenite Group (3) as double dose induced Sodium selenite, Group (4) as multiple dose induced Sodium selenite. Animals were sacrificed and different regions such as Brain, Liver, Kidney and Testis were immediately isolated, frozen in liquid nitrogen and were stored at -400C until analyses. The selected enzymes such as Lactate dehydrogenase (LDH), Malate dehydrogenase (MDH), Succinate dehydrogenase (SDH), Glutamate dehydrogenase (GDH), Adenosine triphosphatases (ATPases), Aspartate aminotransferase (AAT) and Alanine amino transferases (ALAT) were examined in the experimental animals. The present study revealed the toxic effect of selenium in different doses.

PAGES: 72-76  |  168 VIEWS  254 DOWNLOADS