Green Synthesis of Silver Nanoparticles and Antibacterial Studies with Leaf Aqueous Extract of Pittosporum napaulense (DC.) Rehder & E.H Wilson.

Author(s): Arunagiri Usha and Nimmanapalli Yasodamma

Abstract: In the present study we report the green synthesis of stable silver nanoparticles (SNPs) from aqueous leaf extract of P. napaulense. The colour change from grey to brown is observed upon synthesis at 428 nm peaks obtained from UV-VIS spectroscopic analysis. Zeta potential at -13.2 mV, negative value indicates the high stability of nanoparticles. FTIR spectroscopic studies confirms that phenols and proteins of leaf extract is main responsible for capping and stabilization of these SNPs. Crystallographic studies from XRD indicated the SNPs are crystalline in nature. High resolution and magnification studies with TEM analysis revealed that the nanoparticles are spherical in shape having the size range from 8 to 18 nm. EDAX pattern of synthesized SNPs showed 78.86 weight percentage of Ag metal in the sample indicates the purity of sample. Further, the antimicrobial studies of SNPs show high toxicity towards different bacterial isolates as Bacillus subtilis with 18.5 mm diameter zone of inhibition followed by staphylococcus aureus, Escherichia coli and Klebsiella pneumonia. The results revealed that the selected medicinal plant possess the potentiality towards the synthesis of narrow range nanoparticles also combat with the pathogens.

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