Evaluation of Methanol Extract of Tephrosia villosa For in vitro Anticancer and Antioxidant Potentials Against Cancer Cell Lines (Research Article)

Author(s): Soma Pramanik, Pinkey Rawal and Ramesh C

Abstract: Objective: The present study was designed to evaluate methanol extract of Tephrosia villosa for anticancer against cancer and normal cells and also determine its antioxidant properties. Methods: The methanol extract of Tephrosia villosa was prepared using sox-let apparatus. The methanol extract was evaluated for its in-vitro anticancer potentials against human Vero (normal) MCF-7 (breast), HCT-116 (colon), liver (HEP-G2) and A-549 (lung) cancer cell lines by MTT assay and concentration of the methanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, Superoxide scavenging, Lipid peroxidation and DPPH methods were used and IC50 values of extracts were noted. Results: In the present study, methanol extract of Tephrosia villosa has shown anti-cancer property by exhibiting significant IC50 values against all cancer cell lines MCF 7, HCT-116, HEP-G2 and A-549 but IC50 of methanol extract against vero cell lines was not significant. The extract has also shown significant IC50 values in Superoxide scavenging, Lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid. Conclusion: The results obtained from the present research work suggested that the methanol extract of Tephrosia villosa possess significant in vitro cytotoxic potentials against cancer cells MCF 7, HCT-116 and A-549 cell lines but cytotoxic property of extract against normal cell was not significant. The results also suggested that the methanol extract have in vitro antioxidant properties.

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