Formulation and Optimization of Albumin Microspheres for Controlled Delivery of Gene (Research Article)

Author(s): Suresh S*, Dhara C and Malay C

Abstract: In this study, albumin microsphere was formulated for microencapsulation of pBR322 plasmid vector which is believed to be a good vehicle of gene. Controlled release system using biocompatible or biodegradable polymers offer many advantages over conventional gene delivery approaches. The pBR322 plasmid loaded microsphere for targeted gene delivery of tumor was prepared by solvent evaporation technique. Albumin was derived from natural biological source (Biopolymer), Biodegradable, as well as well absorbed in tumor tissue. So, Albumin was selected polymer of dosage forms. We formulated and optimized concentration of albumin as well as DNA which will carry and deliver maximum amount of DNA in control manner. Three different concentration of Albumin with three different concentration of DNA were prepared and evaluated for DNA carrying and delivering efficacy. In future, this formulation may play huge role for huge gene delivery successfully to a tumor tissue with great safety.

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