Green Synthesis of Silver Nanoparticles from Leaf Extract of Calotropis Gigantea and Evaluation of Their in vitro Antibacterial Properties Against Multidrug Resistant Bacteria (Research Article)

Author(s): Vijayalakshmi MV, Usha Rani G, Lakshmi Devi B, Arundathi M, Sowjanya MGS and Ramesh B

Abstract: In recent years, green synthesis approaches for nanoparticles preparations are gaining a lot of attention in various fields including medicine, drug delivery, pharmaceuticals, and agriculture. Their preparation is relatively simple, inexpensive and represents eco-friendly properties. Due to the unprescribed and excess use of antibiotics has led the development of multidrug resistant (MDR) bacteria. Several studies are evidenced that green nanoparticles have potential bactericidal activity. In this study we have prepared silver nanoparticles from Calotropis gigantea leaf extract (Cg-AgNPs) and studied their in vitro antimicrobial activity against MDR Gram negative bacterial pathogens of septic wound infections such as Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The particles characterization was made by using Dynamic Light Scattering experiment (DLS) and Scanning electron Microscope (SEM). The results of the present study revealed that the size of the Cg-AgNPs ranged between 30-70 nm with spherical shapes and showed a moderate antibacterial activity against MDR bacteria.

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