Identification of Molecular Markers to Detect Somaclonal Variations in Banana cv. Grand Naine (AAA) Using Inter Simple Sequence Repeats (ISSR) Molecular Marker Technique (Research Article)

Author(s): Prathibha K. Y, Ravishankar K. V, Shubha, Manjula A.C, and Keshamma E

Abstract: The present study was undertaken with the main purpose of detection of variations of somaclones in the banana cv. Grand Naine by using inter simple sequence repeats (ISSR) molecular marker technique. Matured leaves that are free from damage caused by pests or disease were collected in brown covers from different field places like Anekal, Kadur, Hulimavu, Hesaraghatta. Source of these micropropagated planting materials were from Khodays Biotech, MSR Biotech, Green earth, Ramco Biotech companies. Isolation of genomic DNA was carried out by cetyl trimethyl ammonium bromide. The integrity and purity of DNA and visualization of amplified products was done by electrophoresis and quantified. PCR amplification of DNA from banana somaclones using ISSR markers were carried out using Taq DNA polymerase and short stretch of oligonucleotides (primers), which are specific to the DNA to be amplified. Then the ISSR, markers are used to assess the genetic variation in somaclonal variants of banana cv. Grand Naine. The amplified products of ISSR markers were separated using PAGE electrophoresis technique. Out of 98 ISSR primers screened, 68 primers did not yield any amplified DNA profile and only 34 primers showed amplification. In those 34 primers only 9 showed polymorphism. Overall, 85 monomorphic bands were seen, and 15 polymorphic bands could be seen. In conclusion, overall, 17.6% between dwarf off type and true to type normal banana cv. Grand Naine plants.

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