In vitro α-Amylase, α-Glucosidase and β-Galactosidase Inhibitory Activity of the Extract and Solvent Fractions of Blumea oblique (L.) Druce.

Author(s): Shirumalla Anuradha and Vanapatla Swaroopa Rani*

Abstract: OBJECTIVE: To investigate the therapeutic effects of Blumea oblique methanolic extract and its fractions for α-amylase, α-glucosidase and β-Galactosidase inhibitory activity by in vitro assays. METHODS: Aerial parts of Blumea oblique were extracted with methanol by maceration. And its fractions were prepared by dispersing uniformly in distilled water and subjected to solvent fractionation with toluene, ethyl acetate and butan-2-one. The fractions were concentrated under reduced pressure. The inhibitory effect of these BO extract and its fractions on α-amylase, α-glucosidase and β-galactosidase enzymes was determined by in vitro assays. RESULTS: The results revealed that the methanolic extract and its fractions of Blumea oblique were inhibited α-amylase, α-glucosidase and β-galactosidase enzymes in a dose dependent manner. Among all these fractions, ethyl acetate fraction of Blumea oblique has shown the prominent α-amylase, α-glucosidase and ꞵ-galactosidase enzyme inhibitory activity with IC50 values of 5.74±0.18, 6.16±0.27 and 11.81±0.13mg/ml and it was well comparable with standard IC50 value 5.25±0.41, 5.39±0.28 and 10.72±0.23mg/ml respectively. And Phytochemical reports of Blumea oblique revealed that it contains alkaloids, carbohydrates, steroids, saponins, flavonoids and phenolic compounds. These results substantiate the use of Blumea oblique as medicine for the treatment of diabetes by controlling postprandial hyperglycemia. CONCLUSION: The results suggest that ethyl acetate fraction of aerial parts of Blumea oblique shown significant α-amylase, α-glucosidase and β-galactosidase enzyme inhibitory activity, which may be helpful to reduce the postprandial blood glucose levels. However, the chemical compounds responsible for the inhibitory action of these enzymes need to be further identified and characterized. This may be useful for the development of new antidiabetic agents from plant sources.

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