Establishing and Quantifying the Nitrogen Fixing Potential of the Brinjal Bacterial Isolate (Research Article)

Author(s): Jenifer Lolita C, Manjula A. C, Prathibha K.Y and Keshamma E

Abstract: Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To quantify the amount of nitrogen fixing bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of nitrogen fixing bacteria. Nitrogenase activity was estimated by acetylene reduction assay and analyzed by gas chromatography. The amount of nitrogen fixed brinjal bacterial isolate was quantified by micro Kjeldahl method. The amount of nitrogen fixed by the BBI was equivalent to 23.5 nm of C 2H2 reduced/tube/hour. The amount of nitrogen fixed by the BBI showed a steady increase up to three days (75 nm of C2H2 reduced/tube/hour) after which there was a decline in the amount of nitrogen fixed by the microbe. For the first time the presence of nitrogen fixing bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.v

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