Radical Scavenging Activity of Peptide Fraction from Curcuma pseudomontana L. A Wild Variety Of Turmeric (Research Article)

Author(s): Syeda Hajira Banu, Dugganaboyana Guru Kumar and Mukunda Chethan Kumar

Abstract: The present investigation focusses on antioxidant activity of peptide fraction P4 from a wild variety of turmeric, Curcuma pseudomontana L. (Zingiberaceae). P4 was purified by standard protein purification protocol. 80% saturated ammonium sulphate protein precipitate exhibited 78±2.65% hydroxyl radical scavenging activity, was purified on Sephadex G-75 column that resulted in two peaks labelled as AS1 and AS2. AS2 showed 75±0.76% hydroxyl radical scavenging activity and AS1 exhibited 40±1.23% at 100μg/ml each compared to the reduced glutathione and butylated hydroxyltoluene. AS2 on preparative rp-HPLC eluted at different retention time into four fractions labelled as P1, P2, P3 and P4. The peptide fraction, P4 eluted at 39.73min showed 65±0.88% hydroxyl radical scavenging activity. Peak fractions 1, 2, and 3 exhibited 48±0.98%, 42±1.34% and 49±0.67% hydroxyl radical scavenging activities respectively. P4 efficiently quenched 2,2-diphenyl-1-picrylhydrazyl radicals by 68±0.94%. The ferric ion reducing ability of P4 was concentration dependent which showed 79±1.04% at 25μg/ml. P4 showed lipid peroxidation inhibition activity in a linoleic acid model system.

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