In vitro gametophyte development of Pteris vittata L., Phymatosorous nigrescens (BI.) Pichi-Serm. and Nephrolepis acutifolia (Desv.) H. Chris (Research Article)

Author(s): *R. Kavitha, S. Sahaya Sathish, S. Senthil kumar, S. Bavya and V. Thangarajan

Abstract: The in vitro technique is an art of growing plants in sterile condition to propagate and multiply the rare, endangered and endemic species. Pteris vittata L., Nephrolepis acutifolia (Desv.) H. Chris., Phymatosorous nigrescens (BI.) Pichi-Serm. were collected from Kolli hills for in vitro multiplication. All these ferns are in under conservation status, so these ferns have to conserve for its biological activity and economical value. Matured spores of the 3 varieties of ferns were collected from air dried sporophylls and used as explant. Totally 3 nutrient medium were used such as Murashige & Skoog, 1962 (half strength), Fern micropropagation and Knudson C Orchid medium with different concentration of growth regulators. Spores were germinated after 10days, 30 days, 45 days from the day of inoculation respectively. The fern micropropagation medium shows best results and Pteris vittata fern shows high percentage of spore germination and prothallus development in all the medium used. In Pteris vittata the fern micropropagation medium with 0.4mg/l KIN + 0.2 mg/l GA3 (F2) shows the maximum level of spore germination & protonema initiation (90%) and shows 84% of prothallus development. In Nephrolepis acutifolia, the fern micropropagation medium with 0.4 mg/l KIN + 0.2 mg/l GA3 (F6) showed the maximum level of spore germination & protonema initiation that is 56% and shows 42% of prothallus development. In Phymatosorus nigrescens, the fern micropropagation medium with 0.4 mg/l KIN + 0.2 mg/l GA3 (F9) showed the maximum level of spore germination & protonema initiation (48%) and prothallus development (40%).

PAGES: 236-241  |  182 VIEWS  228 DOWNLOADS