Orchid Mycorrhizal Studies of Nervilia crociformis (Research Article)

Author(s): Jyothsna B.S, Kavitha K.R., and Keshamma E

Abstract: Orchid mycorrhizal associations involve a plethora of distinctive nutrient transport systems, structures and phenomena which have only been observed in the family Orchidaceae. Orchid mycorrhizae are symbiotic relationships between the roots of plants of the family Orchidaceae and a variety of fungi. Hence, current study aimed to isolate and purify the mycorrhizal fungi of orchid from natural habitats of Western Ghats. The terrestrial orchid Nervilia crociformis, was collected from natural habitats from various locations of Kemmangundi and Sagar, Karnataka State and were investigated for mycorrhizal association. Freshly collected root/tuber of Nervilia crociformis was used for isolation, identification and purification. The fungal isolates were purified by hyphal tip method. Classical identification techniques used in the identification of fungi include colony characters and microscopic characters of hyphae and spores. One fungal isolate was obtained from the tuber of Nervilia crociformis which was identified as Colletotrichum fruticola.

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