Thymoquinone Loaded Solid Lipid Nanoparticles Exhibit Cytoprotective Effect Against 3-Nitropropionic Acid Exposed Changes In SHSY-5Y Neuroblastoma Cells (Research Article)

Author(s): Surekha Ramachandran and Sumathi Thangarajan

Abstract: The present study investigated the cytotoxic events upon 3-Nitropropionic acid (3-NP) exposure and the protective effect of thymoquinone nano formulation (TQ-SLNs) in SH-SY5Y neuroblastoma cells. The SH-SY5Y neuroblastoma cells were incubated separately for 1 hr. with TQ-SLNs (0.4 μM) and TQ (5 μM) in 96-well plates, and then the cells were exposed to 3-NP (0.5 mM) for 24 hrs. Exposure of 3-NP (0.5 mM) reduced the cell viability and antioxidant GSH level with a concurrent increased release of LDH in the cytosol. 3-NP exposure also causes loss in Mitochondrial Membrane Potential (MMP) with increased content of cytosolic cytochrome c. The apoptotic incidence upon 3-NP exposure was also evident as an increased nuclear condensation in Hoechst 33342 and Acridine orange/Ethidium bromide staining. On the other hand, TQ-SLNs treatment effectively increased the percentage of cell viability, GSH level, preserved the MMP, controlled the LDH and cytochrome c release, and reduced the nuclear condensation. TQ-SLNs also found to regulate the cell cycle process, which was found arrested at G0/G1 phase during 3-NP exposure. From the result, we suggest that TQ-SLNs could effectively exhibit beneficial action against cell injury during 3-NP challenged condition.

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