FORMULATION AND CHARACTERIZATION OF TEMOZOLOMIDE LOADED CHITOSAN NANOPARTICLES

Author(s): K Ramakrishna* and K.V. Ramana Murthy

Abstract: The aim of current study was to encapsulate Temozolomide into chitosan-based nanoparticles using a modified ionic gelation method. Different ratios of Temozolomide to chitosan were prepared. The highest encapsulation efficiency of Temozolomide into chitosan nanoparticles was found to be about 46-53 %. The prepared nanoparticles were characterised for particle size analysis and zeta potential. The values of zeta potential of TMZ loaded nanoparticles ranged from -21.7 to 30.1 were sufficient to keep the particles stable. polydispersity indices PDI of all the nanoparticles was in the range of 0.187 to 0.229 indicating all the formulation were homogenous in distribution. In vitro drug release studies showed initially rapid drug release was observed due to surface attached drug that varies from 28 to 37 % and later on the drug release was consistent in all the formulations, The drug release was studies for 48 hours and the release was found to be around 90% from all four formulations. The in vitro release study revealed that the release of Temozolomide could be better sustained from chitosan nanoparticles. Drug and excipient compatibility was established by DSC nd FTIR study and showed no drug polymer interaction. Surface morphology of nanoparticles were characterized by SEM study the prepared nanoparticles are separate and free flowing and spherical in nature without any aggregates.

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