Evaluation of Antiulcer Activity of Ethanolic Extract of Tubers of Gloriosa superba Linn in Albino Rats (Research Article)

Author(s): A N Shifila* and Varkey Joyamma

Abstract: Aim: The Plant Gloriosa superba Linn has been reported for a variety of ethnic medicinal uses. The present study aimed to evaluate the antiulcer activity of ethanolic extract of tubers of Gloriosa superba Linn (EEGS) in albino rats. Methods: Antiulcer activity was evaluated using Pylorus ligation induced ulcer animal model in albino rats. The dried and powdered tubers of Gloriosa superba were successively extracted with petroleum ether and ethanol. The ethanolic extract was subjected to phytochemical screening to identify various phytoconstituents. The LD50 value of EEGS was found to be 1260 mg/kg. The Volume, pH, total acidity and free acidity of gastric fluid, ulcer score, ulcer index and percent inhibition of ulcer were measured. Data were analyzed using One-way Analysis of Variance followed by Tukey’s post hoc test, and P<0.05 was considered as statistically significant. The test group received two doses (80mg/kg and 120mg/kg) of the extract for seven days before induction of the ulcer. Positive control group received the standard drug Omeprazole(20mg/kg) and negative control group received vehicle (1% CMC). Results: Preliminary phytochemical screening of the extract showed the presence of carbohydrates, alkaloids, steroids, flavanoids, tannins and phenolic compounds. The antiulcer activity of EEGS was evidenced by the significant attenuation of gastric volume, pH, free acidity, and total acidity in the gastric juice of pyloric-ligated rats in a dose-dependent manner. Conclusions: The results of the present study suggest that EEGS possessed significant dose dependent antiulcer effect in Pylorus ligated ulcer model.

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