Analytical Method Development and Validation for The Simultaneous Estimation of Azithromycin and Levofloxacin by RP-HPLC (Research Article)

Author(s): A. Deepa*, P. Raj kumar, G. Sravanthi, M. Mamata and S. Divya

Abstract: A new method was established for simultaneous estimation of Azithromycin and Levofloxacin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Azithromycin and Levofloxacin by using ZODIAC –SIL RP C18 column 4.6×100 mm 3.0μm, flow rate was 1.0 ml/min, mobile phase ratio was (75:25 v/v) acetonitrile:phosphate buffer (KH2PO4 and K2HPO4) pH 9 ( pH was adjusted with orthophosphoric acid), detection wave length was 292 nm. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study for Azithromycin and Levofloxacin was found in concentration range of 6.25μg-37.5μg and 0.5μg-3.0μg and correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 99.56% and 99.48%, %RSD for repeatability was 0.27 and 0.40, % RSD for intermediate precision was 0.27 and 0.94 respectively. The precision study was precise, robust, and repeatable. LOD value was 2.17 and 6.60, and LOQ value was 0.032 and 0.1125 respectively.

PAGES: 68-72  |  185 VIEWS  201 DOWNLOADS