Development and Characterization of Chitosan-Alginate based Low Molecular Weight Heparin Novel Nanoparticles for Oral Delivery (Research Article)

Author(s): Satheesh Jogala, Shyam Sunder Rachamalla, Jithan Aukunuru

Abstract: Objective: The objective of the current research work was to prepare and evaluate novel chitosan-alginate based nanoparticles for oral delivery of low molecular weight heparin (LMWH). In this study, we prepared orally administered nanoparticles encapsulating LMWH using chitosan-alginate by employing Polyelectrolyte complexation method. The formulated nanoparticles were evaluated for size, shape, zeta potential, in vitro drug release, and in-vivo biological activity (anti factor Xa activity) using standard kit. Scanning electron microscopic (SEM) studies on the nanoparticles confirmed the formation of spherical particles with smooth surface. The size of the formed nanoparticles was about 415-485nm. The % entrapment of nanoparticles was found to be between 46.5-80.5%. Optimized Nanoparticles formulation showed 93% drug release in 24 hrs. Optimized nanoparticles exhibited excellent improvement in pharmacokinetic parameters when compared to Clexane LMWH marketed product when administered orally. The results of this study revealed that oral nanoparticles are suitable candidates for targeted delivery of LMWH through colon route into systemic circulation.

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