Phytochemical and in vitro Anthelmintic Activity of Aqueous Polyherbal Leaf Extract against Pheretima Postuma (Research Article)

Author(s): Kakunuri Lakshmi*, Adapa Sowmya, G. Subba Rao, Chinnam Kavya, Kodali Shoba Rani, Shaik Sabeeha, Dola Vasantha Kumari and Kareti Ramya

Abstract: The main aim and objective of the study was to evaluate the phytochemical screening and anthelmintic activity of aqueous polyherbal leaves extract (APLE) against Pheretima posthuma. The leaves of Emblica Officinalis, Terminalia bellerica, Carica papaya, Citrus limon and Achras sapota was identified and purchased from local market of Nuzvid. APLE was prepared from the dried leaves of five different plants using the solvent water. Initially, APLE was screened for phytochemical constituents by standard methods. Further, antihelmintic study was conducted against Pheretima posthuma, collected from local Vermicomposting Farm, Nuzvid. In the phytochemical screening, APLE showed presence of glycosides, alkaloids, flavonoids, phenols, phytosterols and tannins. In the anthelmintic study, mortality was produced in earth worm populations by APLE. The use of APLE as an anthelmintic was confirmed by using standard method against Pheretima posthuma. The results indicated that the test drug has significant anthelmintic properties. The data were found statistically significant by using one way ANOVA (P< 0.0001). Hence, it can be concluded that the APLE can be used as a novel drug for the treatment of worm infestations.

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